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Motor Protein Motility
We use advanced microscopy and force spectroscopy techniques to
measure the mechanical properties of molecular motor proteins, with a focus on the kinesin-related proteins that participate in axonal
transport and cell division. Ultimately, we aim to understand how motors work individually and cooperatively to
achieve large-scale motion in healthy and diseased cells.
Trajectory shows kinesin motion along microtubule
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Single Filament Elasticity and Structure
Fluorescently-labeled cytoskeletal filaments are confined to two dimensions
and visualized using a custom-built total internal reflection fluorescence (TIRF) microscope. The flexural rigidity of isolated and protein-coated
filaments is directly determined from thermally-induced shape changes.
Montage of images of microtubules
(Click to play movie) |
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 | | Image of reconstituted cytoskeleton |
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Mechanics of the Cytoskeleton
Cells contain a dynamic and enzymatically active polymer network called the
cytoskeleton that allows them to respond to chemical and mechanical inputs in real time. We seek to understand the molecular origins of cytoskeletal strength and shape using novel microscopy and micromanipulation
techniques.
Our results will advance our understanding of important force-sensitive biological processes, inclduing stem cell differentiation and
wound healing. More generally, our experiments will provide new insight into the physics of self-assembled systems and will enable the development
of novel bio-inspired materials. |
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Predictive Modeling of Biological Materials
We apply traditional engineering tools, including stochastic modeling and
finite element analysis, to understand how forces are generated and transmitted in soft biological materials.
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 | | Monolayer of human
cervical cancer cells |
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Force Generation by Living Cells
Using microscopy and
force spectroscopy techniques, we examine the effect of substrate chemistry and mechanics on cell growth and movement. Ultimately, we aim
to understand how forces regulate large-scale motion in healthy and diseased tissues, and use that information to develop improved
diagnostics and clinical treatments for diseases such as Alzheimer’s and cancer.
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Instrument Development
We are developing a number of novel imaging and force spectroscopy techniques, as well as specialized devices for micro- and nanoscale
manipulation of single proteins, filaments, and cells. |
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